maxsep magnetic separator

A Phase I Trial of CD3/CD28-activated T Cells (Xcellerated ...

moved by processing over a MaxSep Magnetic Separator (Nex-ell Therapeutics, Irvine, CA). Next, the T cells were activated and culture-expandedex vivo in a closed system in an adaptation of a process described previously (10). Briefly, the T cells were costimulated

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Efficient clinical-scale enrichment of lymphocytes for use ...

2009-1-1  As reported here and by collaborators , monocyte depletion can be achieved by incubating PBMC with Dynabeads, non-porous polystyrene superparamagnetic beads, in the absence of specific antibodies, by capture of adherent cells and removal via a large-scale MaxSep or ClinExVivo ™ magnetic separator. The MaxSep ‘semi-closed’ method requires ...

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Purging in BCR-ABL-positive acute lymphoblastic leukemia ...

Dynabeads (Dynal, Hamburg, Germany) and the Baxter MaxSep Magnetic cell separator (Baxter, Deerfield, IL, USA) were used for IMB purging in all patients. From 1992 to 1996, a triple cocktail of ...

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Adoptive transfer of costimulated T cells induces ...

2003-9-15  The magnetic beads were removed using a Baxter Fenwal Maxsep magnetic cell separation system. After cell culture, harvest was performed on the day of scheduled cell reinfusion with the Baxter Fenwal cell harvester; the cells were washed and resuspended in Plasmalyte A containing 1% HSA.

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Safety, tumor trafficking and immunogenicity of chimeric ...

2017-3-21  On Day 3, the beads were removed using a Maxsep magnetic bead separator (Baxter, Roundlake, IL, USA) and resuspended in AIM-V medium with IL-2 (200 IU/mL). Transduction with CC49-ζ retroviral supernatant was performed on Days 5 and 7 in AIM-V medium containing IL-2 and polybrene (Aldrich, St Louis, MO, USA) using spinoculation at 800 x g for ...

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Expression of a Functional c-k/t Receptor on a Subset of ...

Cells were next adhered to a MaxSep Magnetic Cell Separator (Baxter HealthCare Corp., Deerfield, IL) for 10 min. Nonadherent cells were then labeled with directly conjugated mAbs and sorted for CD56 bright, CD56 dim, CD56 bright c-kit receptor-positive

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[18F]FHBG PET/CT Imaging of CD34-TK75 Transduced

2015-6-1  After removing the Dynabeads using a Fenwell MaxSep Magnetic Separator (Baxter, Deerfield, IL), cells were washed in PBS containing 2 mmol/l ethylenediaminetetraacetic acid and 25% human serum albumin, then centrifuged (510 × g, 7 minutes) to concentrate them.

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利用激活的t细胞使抗原提呈细胞成熟的制作方法

2002-4-26  室温1rpm(立式旋转(end-over-endrotation))约30分钟,混合小珠和细胞。将装有小珠和细胞的袋子置于MaxSep Magnetic Separator(Nexell Therapeutics,Irvine,CA)上。在袋子和MaxSep之间放置一个塑料间隔物(约6mm厚)(可以去掉该间隔物来增加磁力的强度)。

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CellCAN

He joined Baxter Healthcare in 1987, where he worked on the development of the MaxSep and Isolex magnetic cell separators. He co-founded the International Society for Hematotherapy and Graft Engineering (ISHAGE, now ISCT), and the Journal of Hematotherapy (now Cytotherapy) in 1992. From 1992 to 1997 he helped establish the stem cell ...

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Our Facilities Clinical Cell and Vaccine Production ...

Baxter MaxSep Magnetic Separator: LN 2 Freezer: Centrifuge, Microcentrifuge: LN 2 Manifold Changeover: Centrifuge, Sorvall Biofuge Primo R: LUNA Cell Counter: Centrifuge, Sorvall Legend RT: MaxCyte Flow Transfection System: Centrifuge, Sorvall Legend XTR: Microscope, Inverted: Centrifuge, Sorvall RC3B-plus: Miltenyi CliniMACS Magnetic Separator

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A Randomized Phase II Trial of Idiotype Vaccination and ...

2021-9-16  The beads were then removed by 148 magnetic separation by the MaxSep Magnetic Separator, and the number of recovered 149 (monocyte-depleted) cells were determined. Cells were then grown in X-VIVO15TM 150 media supplemented with 5% commercial pooled human AB serum. Anti-CD3/anti-151 CD28 antibody coated microbeads (Dynal, ThermoFisher) were ...

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Adoptive transfer of costimulated T cells induces ...

2003-9-15  The magnetic beads were removed using a Baxter Fenwal Maxsep magnetic cell separation system. After cell culture, harvest was performed on the day of scheduled cell reinfusion with the Baxter Fenwal cell harvester; the cells were washed and resuspended in Plasmalyte A containing 1% HSA.

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Expression of a Functional c-k/t Receptor on a Subset of ...

Cells were next adhered to a MaxSep Magnetic Cell Separator (Baxter HealthCare Corp., Deerfield, IL) for 10 min. Nonadherent cells were then labeled with directly conjugated mAbs and sorted for CD56 bright, CD56 dim, CD56 bright c-kit receptor-positive

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Safety, tumor trafficking and immunogenicity of chimeric ...

2017-3-21  On Day 3, the beads were removed using a Maxsep magnetic bead separator (Baxter, Roundlake, IL, USA) and resuspended in AIM-V medium with IL-2 (200 IU/mL). Transduction with CC49-ζ retroviral supernatant was performed on Days 5 and 7 in AIM-V medium containing IL-2 and polybrene (Aldrich, St Louis, MO, USA) using spinoculation at 800 x g for ...

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Control of Epstein-Barr Virus Load and

COBE 2991 blood lymphocyte separator (COBE). The CD4+ and CD8+ T lymphocytes were depleted by immunomagnetic separation in 2 steps. First, bone marrow mononuclear lym-phocytes were incubated with Dynabeads M-450 CD4 (Dynal) for 30 min. The immunomagnetic bead-carrying lymphocytes were then depleted by use of a MaxSep Magnetic Cell Separator ...

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利用激活的t细胞使抗原提呈细胞成熟的制作方法

2002-4-26  室温1rpm(立式旋转(end-over-endrotation))约30分钟,混合小珠和细胞。将装有小珠和细胞的袋子置于MaxSep Magnetic Separator(Nexell Therapeutics,Irvine,CA)上。在袋子和MaxSep之间放置一个塑料间隔物(约6mm厚)(可以去掉该间隔物来增加磁力的强度)。

Read More
CellCAN

He joined Baxter Healthcare in 1987, where he worked on the development of the MaxSep and Isolex magnetic cell separators. He co-founded the International Society for Hematotherapy and Graft Engineering (ISHAGE, now ISCT), and the Journal of

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MAGNETIC SEPARATORS

2019-12-27  omatic magnetic separator for. Examples of application of magnetic separators in various fi elds Removal of iron and collection of iron powder from various kinds of raw materials and semifi nished products are called magnetic separation Kanetec off ers a wide variety of magnetic separators for use with lump materials bulk materials claylike materials and liquids

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Shanghai LIVIC Filtration System Co., Ltd.: Hydraulics ...

Discover all the products from Shanghai LIVIC Filtration System Co., Ltd. and see a list of their distributors. Contact the manufacturer directly for a quote.

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Combined positive and negative cell selection from ...

2001-5-4  Using these magnetic bead separation systems, two groups of sequential +/− selection (Isolex 300i version 1/MaxSep and Isolex 300i version 2/MaxSep) and two groups of simultaneous +/− ...

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ultramag magnetic separator - passofinto.fr

2021-7-13  maxsep magnetic separator. MagneticSeparatorsSpherotech. Themagneticpegs ofUltraMagSeparatorfit between the wells underneath the 96 well plate The microplate is read in an appropriate microtiter plate reader by loading the microplate with the attachedUltraMagSeparatorinto the reader The magnets ensure that themagneticparticles stay out of light ...

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Rapid immune recovery and graft-versus-host disease-like ...

2009-6-9  The beads were removed with a Baxter Fenwal Maxsep® magnetic separator, washed and concentrated with the Baxter Fenwal Harvester System, and resuspended in 100–500 mL 1:1 Plasmalyte A/Dextrose 5%, 0.45% NaCl containing 0.5–1% human serum albumin. The harvested cells were transported by courier from the cell production facility to the ...

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Prolonged survival and tissue trafficking following ...

2000-8-1  On day 3, the beads were removed using a Maxsep magnetic bead separator (Baxter, Roundlake, IL) and resuspended in AIM-V medium with IL-2 and ritonavir. Transduction with CD4ζ retroviral supernatant at a multiplicity of infection of 2 viral particles per cell was performed on days 5 and 7 in AIM-V medium containing IL-2, ritonavir, and ...

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Control of Epstein-Barr Virus Load and

COBE 2991 blood lymphocyte separator (COBE). The CD4+ and CD8+ T lymphocytes were depleted by immunomagnetic separation in 2 steps. First, bone marrow mononuclear lym-phocytes were incubated with Dynabeads M-450 CD4 (Dynal) for 30 min. The immunomagnetic bead-carrying lymphocytes were then depleted by use of a MaxSep Magnetic Cell Separator ...

Read More
Safety, tumor trafficking and immunogenicity of chimeric ...

On Day 3, the beads were removed using a Maxsep magnetic bead separator (Baxter, Roundlake, IL, USA) and resuspended in AIM-V medium with IL-2 (200 IU/mL). Transduction with CC49-ζ retroviral supernatant was performed on Days 5 and 7 in AIM-V medium containing IL-2 and polybrene (Aldrich, St Louis, MO, USA) using spinoculation at 800 x g for ...

Read More
CellCAN

He joined Baxter Healthcare in 1987, where he worked on the development of the MaxSep and Isolex magnetic cell separators. He co-founded the International Society for Hematotherapy and Graft Engineering (ISHAGE, now ISCT), and the Journal of Hematotherapy (now Cytotherapy) in 1992. From 1992 to 1997 he helped establish the stem cell ...

Read More
MAGNETIC SEPARATORS

2019-12-27  omatic magnetic separator for. Examples of application of magnetic separators in various fi elds Removal of iron and collection of iron powder from various kinds of raw materials and semifi nished products are called magnetic separation Kanetec off ers a wide variety of magnetic separators for use with lump materials bulk materials claylike materials and liquids

Read More
CellCAN

CellCAN Bioengineering Leader. Dr. Piret is a Professor in the Department of Chemical Biological Engineering and the Michael Smith Laboratories at the University of British Columbia. He has an Applied Mathematics to Biochemistry Bachelor’s degree from Harvard and a Chemical Engineering doctoral degree from MIT.

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Shanghai LIVIC Filtration System Co., Ltd.: Hydraulics ...

Discover all the products from Shanghai LIVIC Filtration System Co., Ltd. and see a list of their distributors. Contact the manufacturer directly for a quote.

Read More

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